Response of electron transport rate of water stress-affected grapevines: Influence of leaf age

The present study examined the electron transport rate of photosynthesis in response to light for leaves of different age following the imposition of water stress in Vitis vinifera during the growth period when leaf carbon gain is largely used for sugar accumulation into the berries. Vines grown in pots were gradually dehydrated to soil moisture levels of 100, 60, 40 and 25 % of field capacity and at each soil moisture level the photosynthetic light response of selected leaves was recorded using chlorophyll fluorescence analysis. Light response curves (LRC) were established by fitting a model of the form of a non-rectangular hyperbola to the data. Changes in quantum yield (Φ), the convexity of LRC (Θ) and the maximum electron transport rate (JTsat) following adjustment to water stress and as a result of leaf aging, were analysed and their contribution towards the formation of LRCs was assessed. F varied little due to leaf aging and as a result of falling soil moisture content, whereas JTsat declined strongly following soil dehydration and the decline was exacerbated by leaf aging. Such behaviour suggests that biochemical/stomatal limitations played a more important role in determining the water stress-related decline of photosynthesis than biophysical processes of energy transfer and electron transport. Θ of LRCs, under well-watered conditions was highest for basal leaves with a leaf plastochron index (LPI) of about 16.6 but only small changes were obvious during adjustment to water stress. The most important adjustment in total photosynthetic electron flow following the onset of water deficits resulted from a depression in JTsat, and was most obvious in leaves of a basal (LPI 16.6) and medium (LPI 6.9) position on the shoot. Results suggest that young, fully mature leaves are an important source of carbohydrate assimilation during berry ripening

On the rate of convergence of the Hamiltonian particle-mesh method

The Hamiltonian Particle-Mesh (HPM) method is a particle-in-cell method for compressible fluid flow with Hamiltonian structure. We present a numer- ical short-time study of the rate of convergence of HPM in terms of its three main governing parameters. We find that the rate of convergence is much better than the best available theoretical estimates. Our results indicate that HPM performs best when the number of particles is on the order of the number of grid cells, the HPM global smoothing kernel has fast decay in Fourier space, and the HPM local interpolation kernel is a cubic spline

Merging Active and Passive Data Sets in Travel-Time Tomography: The Case Study of Campi Flegrei Caldera (Southern Italy)

We propose a strategy for merging both active and passive data sets in linearized tomographic inversion. We illustrate this in the reconstruction of 3D images of a complex volcanic structure, the Campi Flegrei caldera, located in the vicinity of the city of Naples, southern Italy. The caldera is occasionally the site of significant unrests characterized by large ground uplifts and seismicity. The P and S velocity models of the caldera structure are obtained by a tomographic inversion based on travel times recorded during two distinct experiments. The first data set is composed of 606 earthquakes recorded in 1984 and the second set is composed of recordings for 1528 shots produced during the SERAPIS experiment in 2001. The tomographic inversion is performed using an improved method based on an accurate finite-difference traveltime computation and a simultaneous inversion of both velocity models and earthquake locations. In order to determine the adequate inversion parameters and relative data weighting factors, we perform massive synthetic simulations allowing one to merge the two types of data optimally. The proper merging provides high resolution velocity models, which allow one to reliably retrieve velocity anomalies over a large part of the tomography area. The obtained images confirm the presence of a high P velocity ring in the southern part of the bay of Pozzuoli and extends its trace inland as compared to previous results. This annular anomaly represents the buried trace of the rim of the Campi Flegrei caldera. Its shape at 1.5 km depth is in good agreement with the location of hydrothermalized lava inferred by gravimetric data modelling. The Vp/Vs model confirms the presence of two characteristic features. At about 1 km depth a very high Vp/Vs anomaly is observed below the town of Pozzuoli and is interpreted as due to the presence of rocks that contain fluids in the liquid phase. A low Vp/Vs body extending at about 3–4 km depth below a large part of the caldera is interpreted as the top of formations that are enriched in gas under supercritical conditions

IMPLEMENTING DESKTOP COMPUTING, INFRASTRUCTURE, AND QUALITY OF WORKLIFE

This article presents preliminary findings from a three year longitudinal study on the role of desktop computing in the work of 38 work groups that have integrated computing significantly into their work lives. Both quantitative and qualitative data were collected from surveys and interviews. End-users\u27 participation in the process of implementing desktop computers in work groups is examined as a primary contributor to the quality of their work life, as is the available infrastructure (training, supplies, and consulting) to support computing in the work groups. Two primary implementation processes are examined: top-down and grass-roots: We discuss the quality of work life of computer users along five dimensions: participation in decisions about work, job complexity, expertise and involvement in computing, changes in job enrichment attributed to desktop computing, and changes in work effort attributed to desktop computing. The quality of working life is most improved in work groups that computerize with grass-roots processes and have adequate infrastructure to support their work with computing

Amino acid substitutions within the heptad repeat domain 1 of murine coronavirus spike protein restrict viral antigen spread in the central nervous system.

Targeted recombination was carried out to select mouse hepatitis viruses (MHVs) in a defined genetic background, containing an MHV-JHM spike gene encoding either three heptad repeat 1 (HR1) substitutions (Q1067H, Q1094H, and L1114R) or L1114R alone. The recombinant virus, which expresses spike with the three substitutions, was nonfusogenic at neutral pH. Its replication was significantly inhibited by lysosomotropic agents, and it was highly neuroattenuated in vivo. In contrast, the recombinant expressing spike with L1114R alone mediated cell-to-cell fusion at neutral pH and replicated efficiently despite the presence of lysosomotropic agents; however, it still caused only subclinical morbidity and no mortality in animals. Thus, both recombinant viruses were highly attenuated and expressed viral antigen which was restricted to the olfactory bulbs and was markedly absent from other regions of the brains at 5 days postinfection. These data demonstrate that amino acid substitutions, in particular L1114R, within HR1 of the JHM spike reduced the ability of MHV to spread in the central nervous system. Furthermore, the requirements for low pH for fusion and viral entry are not prerequisites for the highly attenuated phenotype

Dynamic Trees with Almost-Optimal Access Cost

An optimal binary search tree for an access sequence on elements is a static tree that minimizes the total search cost. Constructing perfectly optimal binary search trees is expensive so the most efficient algorithms construct almost optimal search trees. There exists a long literature of constructing almost optimal search trees dynamically, i.e., when the access pattern is not known in advance. All of these trees, e.g., splay trees and treaps, provide a multiplicative approximation to the optimal search cost. In this paper we show how to maintain an almost optimal weighted binary search tree under access operations and insertions of new elements where the approximation is an additive constant. More technically, we maintain a tree in which the depth of the leaf holding an element e_i does not exceed min(log(W/w_i),log n)+O(1) where w_i is the number of times e_i was accessed and W is the total length of the access sequence. Our techniques can also be used to encode a sequence of m symbols with a dynamic alphabetic code in O(m) time so that the encoding length is bounded by m(H+O(1)), where H is the entropy of the sequence. This is the first efficient algorithm for adaptive alphabetic coding that runs in constant time per symbol

Converted phase identification and retrieval of Vp/Vs ratios from move-out reflection analysis: application to the Campi Flegrei caldera

Here, we propose a method for the determination of Vp/Vs ratios in a horizontally layered propagation media using maximization of a coherency function along theoretical travel-times of PS reflected phases. The theoretical travel-times are computed using the information about the propagation media that is extracted by velocity analysis or by topographic analysis performed on the first arrivals. The method is also a valid tool for the identification of the PS phases associated with a fixed seismic reflector, and it is particularly suitable for data that is stored in common mid-point and common conversion point bin- ning; for this kind of data the hypothesis of horizontally and layered media can usually be verified. We applied the method to both simulated and real datasets. The use of the real data that was acquired in the Campi Flegrei caldera (southern Italy) allowed us to estimate a relatively high Vp/Vs ratio (3.5 ± 0.6) for a very shallow layer (maximum depth, 600 m). This hypothesis has been tested by theoretical rock physical modeling of the Vp/Vs ratios as a function of porosity, suggesting that the shallow layer appears to be formed of unconsolidated, water-saturated, volcanic and marine sediments that filled Pozzuoli Bay during the post-caldera activity

On the pathogenesis of penile venous leakage: role of the tunica albuginea

<p>Abstract</p> <p>Background</p> <p>Etiology of venogenic erectile dysfunction is not exactly known. Various pathologic processes were accused but none proved entirely satisfactory. These include presence of large venous channels draining corpora cavernosa, Peyronie's disease, diabetes and structural alterations in fibroblastic components of trabeculae and cavernous smooth muscles. We investigated hypothesis that tunica albuginea atrophy with a resulting subluxation and redundancy effects venous leakage during erection.</p> <p>Methods</p> <p>18 patients (mean age 33.6 ± 2.8 SD years) with venogenic erectile dysfunction and 17 volunteers for control (mean age 31.7 ± 2.2 SD years) were studied. Intracorporal pressure was recorded in all subjects; tunica albuginea biopsies were taken from 18 patients and 9 controls and stained with hematoxylin and eosin and Masson's trichrome stains.</p> <p>Results</p> <p>In flaccid phase intracorporal pressure recorded a mean of 11.8 ± 0.8 cm H₂O for control subjects and for patients of 5.2 ± 0.6 cm, while during induced erection recorded 98.4 ± 6.2 and 5.9 ± 0.7 cmH₂O, respectively. Microscopically, tunica albuginea of controls consisted of circularly-oriented collagen impregnated with elastic fibers. Tunica albuginea of patients showed degenerative and atrophic changes of collagen fibers; elastic fibers were scarce or absent.</p> <p>Conclusion</p> <p>Study has shown that during erection intracorporal pressure of patients with venogenic erectile dysfunction was significantly lower than that of controls. Tunica albuginea collagen fibers exhibited degenerative and atrophic changes which presumably lead to tunica albuginea subluxation and floppiness. These tunica albuginea changes seem to explain cause of lowered intracorporal pressure which apparently results from loss of tunica albuginea veno-occlusive mechanism. Causes of tunica albuginea atrophic changes and subluxation need to be studied.</p